Growth curve of young and aged BALB/cCrSlcNifdc mice. One-month-old mice were grouped (50 males and 50 females, respectively). Body weight was measured on the same day of every months and lasted for 17 months. The lowest and highest values of mouse body weight in the table were calculated from the mean ± SD.

Analysis of spleen, blood and lymph nodes immune cell subpopulations by flow cytometry. Percent of T cells, B cells, NK cells, dendritic cells, neutrophils, monocytes, macrophages, CD8+ T cells, CD4+ T cells, and Tregs in the young BALB/c mice (n=3, 2-month-old) and aged BALB/c mice (n=3, 18-month-old), differentiation or distribution of these cell types in spleen(A), blood(B) and lymph nodes(C). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.