RenMice-antibody Discovery Engine

RenLite

RenLite mice have full human antibody heavy chain variable regions and a common light chain human variable region for the discovery of fully human bsAb/bsADC antibodies.

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  • Key Features of RenLite
  • Validation Data

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    Key Features
    1. Complete human heavy chain variable domain combined with a common light chain substitution in situ
    2. Fully human heavy chain antibody repertoire diversity
    IGH-V (>1%)

    Heavy chain IGHV germline usage of naive RenLite™ mice

    IGH-V (<1%)

    Heavy chain IGHV germline usage of naive RenLite™ mice

    IGHD

    Heavy chain IGHD germline usage of naive RenLite™ mice

    IGHJ

    Heavy chain IGHJ germline usage of naive RenLite™ mice

    3. Human-like CDR3 features
    CDRH3 Length (Amino Acids)

    The median length of heavy chain CDR3 (14aa) from naive Renite™ mice is comparable to the average length of human CDR3.

    Heavy Chain CDR3 Amino Acid Composition

    Human-like CDR3 features, such as increasing frequency of tyrosine usage, are observed in RenLite™ mouse heavy chain CDR3.

    4. Robust immune response comparable to wild type mouse
    Validation Data
    1. Spleen B Cell Development

    Serum concentrations of IgA, IgM and IgG subtypes of RenLite™ and wild type mice were measured by ELISA. Sera were equally diluted between two groups of mice