Inflammation and Autoimmunity

Pruritus

Biocytogen has established MC903-induced and human IL-31 (hIL31)-induced pruritus mouse models, which serve as reliable platforms for studying itch mechanisms and conducting preclinical evaluation of novel antipruritic therapies. These pruritus mouse models are valuable tools for testing drug efficacy in conditions involving IL-31–mediated itch and inflammatory skin diseases.

on this page

Related Resources

    Pruritus Mouse Model
    Pruritus, or itching, is a common and distressing symptom that significantly affects quality of life in both humans and animals. In preclinical research, pruritus in mice serves as a critical model for understanding the underlying mechanisms of itch and for developing novel antipruritic therapies. It can be induced through various approaches, including the application of pruritogens or the use of genetically modified mouse models with altered itch sensitivity.
    Mouse models of pruritus are highly valuable due to the conserved biological pathways of itch between humans and mice, particularly in the study of chronic pruritus related to eczema (atopic dermatitis), psoriasis, and allergic conditions. These models offer important translational potential by helping identify new therapeutic targets and accelerating itch-related drug development.
    MC903-Induced Pruritus Mouse Model
    Establishment of MC903-Induced Pruritus Mouse Model
    • Experimental animals: C57BL/6, B-hIL31/hIL31RA/hOSMR mice, female, 8-10 weeks old
    • Modeling reagent: MC903 (Calcipotriol)
    • Modeling method: Mice received MC903 (2 nmol/20 μL) on right ear every two days for 9 times. Camera was used to record behavior for 2 times, 1 hour every time. The video is recorded to assess their itching behavior.
    Readouts
    Included tests Body Weight
    Scraching bouts for 60 min post hIL31 injection
    Optional tests Clinical scores Ear thickness
    Skin histopathology (H&E) Immune cell infiltration
    Epidermal thickness
    Histology score
    Molecular levels Serum total IgE
    Behavior Pruritus
    Efficacy Evaluation of Nemolizumab (Anti-IL31Rα) in MC903-Induced Pruritus Mouse Model Using IL31/IL31RA/OSMR Humanized Mice (B-hIL31/hIL31RA/hOSMR Mice)
    Efficacy evaluation of nemolizumab (anti-IL31Rα) in MC903-induced pruritus mouse model using B-hIL31/hIL31RA/hOSMR mice. Scratching bouts in 60 min on Day 14 (A) and Day 17 (B).
    Related Products
    Product Name Product No. Background Action
    B-hCCL24 mice 111396 C57BL/6N
    B-hF2RL1 mice 112362 C57BL/6
    B-hGRP mice 111902 C57BL/6
    B-hIL13/hIL13RA1 mice 120556 C57BL/6
    B-hIL31/hIL31RA/hOSM/hOSMR mice 112647 C57BL/6
    B-hIL31/hIL31RA/hOSMR mice 130746 C57BL/6
    B-hIL31/hIL31RA/hOSMR/hIL4/hIL4RA mice 112908 C57BL/6
    B-hOSM/hOSMR mice 121329 C57BL/6
    MORE >>