Biocytogen has established ovariectomy-induced osteoporosis models in various strains, including C57BL/6 mice, target-humanized mice such as B-hRANKL, and rats. These models are utilized to simulate menopausal osteoporosis for the evaluation of drug efficacy.
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Osteoporosis is a systemic bone disease caused by a variety of reasons. It is characterized by decreased bone density and bone quality, damage to bone microstructure, and increased bone fragility, which is prone to fractures. Estrogen can affect bone metabolism. After menopause, the level of estrogen decreases and cannot effectively inhibit osteoclasts, resulting in active osteoclasts, rapid decomposition and absorption of bone cells, reduced bone mass and accelerated bone loss, leading to osteoporosis. Bilateral ovariectomy in mice was used to simulate menopausal osteoporosis for evaluation of drug efficacy.
| Detection indicators | |
| Micro-CT | Ex Vivo Left Femur |
| HE | Bone Trabecula Area |
Ovariectomy induced osteoporosis in mice. A. C57BL/6 mice were received ovariectomy on day 0. Body weight were recorded every week. Six weeks after operation, uterus and bones were harvested and tested. B. Ovariectomy produced a loss in bone trabecula and uterus weight while an increase in body weight. Values were expressed as mean ± SEM. N = 8, T-test, * p<0.05, ****p<0.0001.
Ovariectomy induced osteoporosis in mice. C57BL/6 mice were received ovariectomy on day 0. Six weeks after operation, bones were harvested and imaged with Micro-CT. Ovariectomy produced a bone loss. Values are expressed as mean ± SEM. N = 8, T-test, * p<0.05, **p<0.01, ***p<0.001.
| Detection indicators | |
| Micro-CT | Left Femur and Tibia |
Ovariectomy induced osteoporosis in B-hRANKL mice. A. In vivo CT were performed in B-hRANKL mice 5 weeks after OVX. The results showed that the bone density of mouse tissues decreased after OVX. B. In vivo CT were performed in B-hRANKL mice 7 weeks after OVX. The results showed that Denosumab increased the tissue bone density of OVX mice. C. Ex vivo CT were performed in B-hRANKL mice 10 weeks after OVX. The results showed that Denosumab increased tibial bone density in OVX mice. Values were expressed as mean ± SEM. Two group were analyzed with T-test, three group were analyzed with one way ANOVA. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
| Detection indicators | |
| Micro-CT | Left Femur and Tibia |
| ELISA | Blood ALP (alkaline phosphatase) |
Ovariectomy induced osteoporosis in rat. A. SD rats were received ovariectomy (OVX) on day 0. B. Body weight was recorded every week. C. Two weeks after operation, ALT in serum was tested. D-E. In vivo CT was performed to analyze the state of bone in rat. Values are expressed as mean ± SEM. N = 5-10, T-test, * p<0.05, **p<0.01, ***p<0.001.
Ovariectomy induced osteoporosis bone loss in rat. SD rats were received ovariectomy (OVX) on day 0. Eight weeks after OVX, animals were sacrificed for femur and tibia collecting. Ex vivo CT was performed for bone structure and mineral density analysis. mean ± SEM. n=5-10, T-test, * p<0.05, **p<0.01, ***p<0.001.
