Inflammation and Autoimmunity

Osteoporosis

Biocytogen has established ovariectomy-induced osteoporosis models in multiple species and strains, including C57BL/6 mice, target-humanized mice such as B-hRANKL, and rats. These well-characterized preclinical osteoporosis models closely mimic menopausal osteoporosis and are ideal for in vivo evaluation of osteoporosis drug efficacy, especially for therapies targeting the RANK/RANKL signaling pathway.

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    Osteoporosis Mouse Model
    Osteoporosis is a systemic bone disease characterized by reduced bone density, impaired bone microarchitecture, and increased bone fragility, making individuals more susceptible to fractures. One major cause is estrogen deficiency, especially after menopause, which leads to increased osteoclast activity, accelerated bone resorption, and progressive bone loss. To mimic postmenopausal osteoporosis in preclinical research, bilateral ovariectomy (OVX) in mice is widely used. This well-established ovariectomy-induced osteoporosis model enables effective in vivo evaluation of osteoporosis drugs targeting bone metabolism and osteoclast-mediated bone degradation.
    Ovariectomy-Induced Osteoporosis Mouse Model
    Establishment of Ovariectomy-Induced Osteoporosis Mouse Model in C57BL/6 Mice
    Detection indicators
    Micro-CT Ex Vivo Left Femur
    HE Bone Trabecula Area
    Establishment of ovariectomy-induced osteoporosis mouse model in C57BL/6 mice. A. C57BL/6 mice were received ovariectomy on day 0. Body weight were recorded every week. Six weeks after operation, uterus and bones were harvested and tested. B. Ovariectomy produced a loss in bone trabecula and uterus weight while an increase in body weight. Values were expressed as mean ± SEM. N = 8, T-test, * p<0.05, ****p<0.0001.
    Ovariectomy induced osteoporosis in C57BL/6 mice. C57BL/6 mice were received ovariectomy on day 0. Six weeks after operation, bones were harvested and imaged with Micro-CT. Ovariectomy produced a bone loss. Values are expressed as mean ± SEM. N = 8, T-test, * p<0.05, **p<0.01, ***p<0.001.
    Efficacy Evaluation of Denosumab in Ovariectomy-Induced Osteoporosis Model Using RANKL Humanized Mice (B-hRANKL Mice)
    Detection indicators
    Micro-CT Left Femur and Tibia
    Efficacy evaluation of denosumab in ovariectomy-induced osteoporosis mouse model using B-hRANKL mice. A. In vivo CT were performed in B-hRANKL mice 5 weeks after OVX. The results showed that the bone density of mouse tissues decreased after OVX. B. In vivo CT were performed in B-hRANKL mice 7 weeks after OVX. The results showed that Denosumab increased the tissue bone density of OVX mice. C. Ex vivo CT were performed in B-hRANKL mice 10 weeks after OVX. The results showed that Denosumab increased tibial bone density in OVX mice. Values were expressed as mean ± SEM. Two group were analyzed with T-test, three group were analyzed with one way ANOVA. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.