Biocytogen has developed Pristane-induced SLE mouse models and MRL/lpr spontaneous SLE mouse models for pharmacological and efficacy evaluation of SLE therapeutics. In addtion, we have generated over 900 human target KI mice covering SLE related targets for testing various drugs.
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Systemic lupus erythematosus (SLE) is a severe autoimmune disease characterized by dysfunction of immune regulation, overproduction of inflammatory cytokines and attack on normal tissues (heart, joints, skin, lungs, blood vessels, liver, kidneys, and the nervous system) by self-reactive cells and antibodies.
Pristane, also known as hydrocarbon oil (2,6,10,14- tetramethylpentadecane, TMPD), is capable of triggering a wide range of autoantibodies. Mice receive priatane develop an ascitic fluid enriched with monoclonal antibodies, local chronic inflammation (lipogranulomas), and a rheumatoid-like erosive arthritis. These clinical manifestations resemble SLE.
Biocytogen has developed Pristane-induced SLE mouse models and MRL/lpr spontaneous SLE mouse models for pharmacological and efficacy evaluation of SLE therapeutics. In addtion, we have generated over 900 human target KI mice covering SLE related targets for testing various drugs.
| Tissue Sample | Evaluation Index |
| Urine | Urine protein/ |
| Serum | Anti-ds DNA antibody |
| Blood biochemical index | UREA |
| CREA | |
| Pathological detection | PAS staining |
| H&E staining |
Content of Proteinuria in urine (A) and mouse Anti-dsDNA IgG in serum (B) were elevated after treatment with Pristane.
Content of mouse Anti-dsDNA IgG in serum was decreased after treatment with anti-CD40 antibody in pristane-induced SLE B-hCD40 Mouse Model.
| Sample | Evaluation Index |
| Urine | Urine protein |
| Serum | Anti-dsDNA antibody, ANA |
| Pathological detection | H&E staining |
| IHC staining | |
| Body weight, Survival curve |
Changes of body weight, urine protein, survival curve, anti-dsDNA and ANA in serum at different time points.
Histological analysis of the kidneys of SLE model.
A. Glomerular hypertrophy, glomerular cell hyperplasia, and increased matrix were observed in kidney; Epidermal cell hyperplasia, epidermal thickening, hyperkeratosis with hypokeratosis, dermal inflammatory cell infiltration were observed in skin (mice aged 20 weeks).
B. C3 and IgG deposition in the kidney of mice aged 20 weeks
| Readout | ||
| Included tests | Survival rate | Survival rate |
| Urine | Urine protein | |
| Serum | UREA | |
| CREA | ||
| Histopathology | HE | |
| Periodic Acid-Schiff stain (PAS) | ||
| Masson staining | ||
| Optional tests | Tissue homogenate | Cytokines test |
| Tissue histopathology | IHC | |
