Inflammation and Autoimmunity

Delayed Type Hypersensitivity

Biocytogen has established OXA- and KLH-induced delayed-type hypersensitivity (DTH) mouse models, which serve as robust and validated platforms for evaluating the efficacy of immunosuppressant drugs in preclinical studies. These models are ideal for assessing anti-inflammatory responses and immune modulation, supporting the development of therapies targeting T cell–mediated immune reactions.

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    Delayed-Type Hypersensitivity (DTH) Mouse Model
    Delayed-type hypersensitivity (DTH) is a type of cell-mediated immune response characterized by mononuclear cell infiltration and tissue damage caused by effector T cells recognizing specific antigens. This delayed hypersensitivity response typically occurs in conditions such as bacterial and viral infections, cancer, transplant rejection, and contact dermatitis.
    The mouse contact hypersensitivity model is widely used to study T cell–mediated immune responses to contact allergens. Oxazolone (OXA), a classic hapten, forms complete antigens when applied to the skin, activating T cells and inducing delayed-type hypersensitivity reactions upon re-exposure after 4–7 days.
    Keyhole limpet hemocyanin (KLH) is a potent immunogenic protein that facilitates dendritic cell–mediated activation of Th1 cells, making it ideal for inducing systemic DTH responses. After initial sensitization, a delayed-type of hypersensitivity reaction is triggered upon local re-challenge, measured by swelling at the injection site.
    Biocytogen has successfully established OXA- and KLH-induced DTH mouse models, offering robust platforms for DTH immunology research and immunosuppressant efficacy evaluation in preclinical studies.
    OXA Induced DTH Model
    Establishment of OXA-Induced DTH Mouse Model
    Efficacy Evaluation of Dexamethasone in OXA-induced DTH Model of BALB/c Mice
    Efficacy evaluation of Dexamethasone in DTH model of BALB/c mice. A. Ear thickness (mm). B. Change of ear thickness (mm). (A. Two-way ANOVA; B. One-way ANOVA; ** p< 0.01 , **** p< 0.0001).
    Histologic assessment of DTH in BALB/c mice. Ear-swelling responses induced by 1% OXA were assessed by measurement of ear thickness (H&E staining). And evaluate the efficacy of anti-TNFα Ab or Dexamethasone (Dex) in the DTH model.