Biocytogen has established active systemic anaphylaxis (ASA) mouse models in mice, which can be used for pharmacodynamic evaluation of Type I hypersensitivity-related drugs.
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Type I hypersensitivity is known as an immediate reaction and involves immunoglobulin E (IgE) mediated release of antibodies against the soluble antigen (food allergies, animal source, environmental factors, Drugs). This results in mast cell degranulation and release of histamine and other inflammatory mediators. In-vitro markers includes Serum tryptase, Eosinophil, IgE, Histamine, basophil activation test (BAT). It involves diseases such as Atopic dermatitis (AD), Allergic asthma, Allergic rhinitis, Allergic conjunctivitis et al.
At Biocytogen, we have established active systemic anaphylaxis (ASA) mouse models in mice, which can be used for pharmacodynamic evaluation of Type I hypersensitivity-related drugs.
| Readout | |
| Included tests | Body temperature, |
| Score | |
| Optional tests | Histamine (ELISA) |
| Mast cell analysis | |
| Mouse IgE (ELISA) | |
| Anaphylactic symptom score | |
| 0 | No clinical symptoms |
| 1 | Repetitive mouth/ear scratching and ear canal digging with hind legs |
| 2 | Decreased activity; self isolation; puffiness around eyes and/or mouth |
| 3 | Periods of motionless for more than 1 min; lying prone on stomach |
| 4 | No response to whisker stimuli; reduced or no response to prodding |
| 5 | Endpoint: tremor; convulsion; death |
OVA induced active systemic anaphylaxis. Mice were immunized with OVA/AL(OH)3 on day 0 and then challenged with OVA (i.v.) on day 14. Rectal temperatures were recorded at 0-90 minutes after OVA injection (A). The OVA challenge resulted in a decrease in temperature (B) and an increase in both the score (C) and serum IgE concentration (D). Data are presented as mean ± SEM, n=3-6.
| Readout | |
| Included tests | Body temperature |
| Score | |
| Optional tests | Human IgE (ELISA) |
| Mast cell analysis | |
| Histamine (ELISA) | |
