Pain models include inflammatory pain models, cancer pain models, neuropathic pain models, etc. Biocytogen has established a CFA-induced inflammatory pain model and a B6-F10 cell-induced cancer pain model for preclinical efficacy studies.
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Pain is an unpleasant sensory and emotional experience caused by tissue damage or underlying tissue damage, and is also a protective defense response of the mechanism to noxious stimuli. The nervous system senses a variety of thermal and mechanical stimuli, as well as environmental and endogenous chemical stimuli. When intense, these stimuli produce acute pain, and in the case of sustained injury, both the peripheral and central nervous system components of the pain delivery pathway exhibit tremendous plasticity, enhancing pain signals and producing hypersensitivity reactions. This can be beneficial when plasticity promotes protective reflexes, but when changes persist, it can lead to chronic pain conditions.
Pain is a common symptom that has an important impact on the quality of life and health of patients, so the development of effective pain drugs is one of the important goals of pharmaceutical research and development. Mouse pain models are an important tool for studying pain mechanisms, evaluating drug efficacy, and exploring pain management methods.
Pain models include inflammatory pain models, cancer pain models, neuropathic pain models, etc. Biocytogen has established a CFA-induced inflammatory pain model and a B6-F10 cell-induced cancer pain model for preclinical efficacy studies.
| Group | Model | Animal | Animal NO. | Treatment | Dose (mg/kg) | Dosing Route | Frequency |
| G1 | PBS | C57BL/6; Female | 6 | PBS | - | i.p. | O2Dx4; Day 1,3,5,7 |
| G2 | CFA (MT 1 mg/ml) | C57BL/6; Female | 6 | PBS | - | i.p. | O2Dx4; Day 1,3,5,7 |
| G3 | CFA (MT 1 mg/ml) | C57BL/6; Female | 6 | carprofen | 5 | i.p. | O2Dx4; Day 1,3,5,7 |
Effects of carprofen on CFA induced inflammatory pain. Mice receive Intraplantar injection of CFA (20 μL) at hind paw (red point in A was the injection site) on day 0 (A). Withdraw threshold was test on day 1, day3 and day6. On day1 and day3, Vonfrey test was done 30min after Carprofen injection. (B). Paw (C) and spinal cord (D) were collected for cytokine analysis in the end. Two-way ANOVA with Dunnett’ test, *P< 0.05, **P< 0.01 with G1. Values are expressed as mean ± SEM, n=6.
| Group | Model | Animal | Animal No. | Treatment | Dose (mg/kg) | Dosing Route |
| G1 | PBS; 20 μL | C57BL/6; Female | 6 | PBS | - | i.p. |
| G2 | B6-F10 1.6 ×105 cell; 20 μL | C57BL/6; Female | 6 | PBS | - | i.p. |
B6-F10 cell induced cancer pain. Mice receive Intraplantar injection of B6-F10 cell at hind paw on day 0. Withdraw threshold was tested on day 7, day14 and day21. (A) Picture of the modeling paw at day 21, (B) Withdraw threshold. Two-way ANOVA with Dunnett’ test, ****P< 0.0001 with G1. Values are expressed as mean ± SEM, n=6.
