Biocytogen has established a T-cell dependent antibody response (TDAR) model in B-hCD40/hCD40L mice, offering valuable insights into the immunotoxicological profiles of various substances, including potential drug candidates.
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T-Cell dependent antibody response (TDAR) is a crucial immunological process that plays a significant role in the generation of high-affinity antibodies in response to specific antigens. This response is essential for effective immunity against many pathogens and is a vital component of vaccine-induced protection. The most commonly used TDAR-like lesions via keyhole limpet hemocyanin (KLH).
In the mouse model of T-cell dependent antibody response, serum samples are collected at predetermined time points to measure specific antibody levels using ELISA or other immunological assays. TDAR assays provide valuable insights into the immunotoxicological profiles of various substances, including potential drug candidates, and are essential for understanding T cell and B cell interactions in health and disease.
| Readouts | ||
| Optional tests | Molecular levels | Serum IgG and IgM |
| Protein level (Elisa or Luminex) | ||
Efficacy evaluation of antibodies in TDAR model of B-hCD40/hCD40L mice. A. Body Weight during treatment. B. Mouse KLH-IgM in Day 7, 14. C. Mouse KLH-IgG in Day 7, 14. (A. Two-way ANOVA; B-C. One-way ANOVA; **** p< 0.0001).
