Inflammation and Autoimmunity

Acute Lung Injury

Biocytogen uses papain, ALT and IL33 to induce acute lung injury (ALI) in mice. This ALI mouse model is widely used to study the underlying mechanisms of ALI and to evaluate potential therapeutic interventions.

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    Acute Lung Injury (ALI) Mouse Model
    Acute lung inflammation can be incited in response to a variety of allergens, chemicals, or pathogens. Alternaria and papain are allergens that can disrupt the airway epithelium, triggering a rapid eosinophilic inflammation mediated by innate lymphoid cell activation. Intranasal administration of alternaria or papain can be used to recapitulate acute lung injury/inflammation in mice.
    The acute lung injury (ALI) mouse model is employed to investigate the mechanisms of acute lung injury and therapeutic interventions. This model is generally induced by administering cytokines, chemical agents (such as lipopolysaccharide, LPS), or physical stimuli to evoke acute lung inflammation. It leads to pathological changes such as pulmonary edema, inflammatory cell infiltration, and impaired gas exchange. Researchers focus on the inflammatory processes in the lungs to explore potential treatment strategies and improve patient outcomes.
    Papain-Induced Acute Lung Injury Mouse Model
    Establishment of Papain-Induced Acute Lung Injury Mouse Model
    • Experimental animals: B-hTSLP/TSLPR, C57BL/6N, 8-10 weeks old, female
    • Modeling reagent: Papain (intranasally, i.n.)
    • Modeling paradigm:
    Readout
    Included tests Bronchoalveolar Lavage Fluid (BALF) Cell numbers of Neutrophils, eosinophils, and macrophages
    Histopathology Bronchial mucus
    Optional tests BALF IL-4, IL-5, IL-13, TARC…
    Lung tissue homogenate IL-4, IL-5, IL-13, TARC…
    Establishment of Papain-Induced Acute Lung Injury Mouse Model in C57BL/6 Mice
    The count of mCD45 and eosinophils as well as percentage of eosinophils in mCD45. The score of inflammatory cell infiltration and bronchial mucus around blood vessels and bronchi of papain-induced acute lung injury mouse model based on C57BL/6 mice. Values are expressed as mean ± SEM. *** p<0.001, **p<0.01, *p<0.05.
    Degree of modeling in different dosage group. In C57BL/6N mice, the PBS control group (G1) showed no significant abnormalities under the microscope. Compared to the control group (G1), the three dosage groups of the papain model (G2-35ug/40uL, G3-50ug/40uL, and G4-75ug/40uL) exhibited varying degrees of vascular and peribronchial inflammatory cell infiltration, mucus in the lung bronchi, and eosinophil infiltration around the lung blood vessels and bronchi, indicating successful modeling. There was no significant difference in the degree of modeling among the three dosage groups.