B-hB7-H4 B16-F10

• 310909

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B-hB7-H3 MC38
B-hB7-H4 MC38

B-hB7-H4 B16-F10

Product nameB-hB7-H4 B16-F10
Catalog number310909
Strain backgroundC57BL/6
AliasesVTCN1, B7S1, B7X, PRO1291, VCTN1
TissueSkin
DiseaseMelanoma
SpeciesMouse
ApplicationB-hB7-H4 B16-F10 cells have the capability to establish tumors in vivo and can be used for efficacy studies.
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  • Description
  • Targeting strategy
  • Phenotypic analysis
  • Tumorigenicity

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      Description

      The mouse B7-h4 gene was replaced by human B7-H4 coding sequence in B-hB7-H4 B16-F10 cells. Human B7-H4 is highly expressed on the surface of B-hB7-H4 B16-F10 cells.

      Tageting strategy
      The exogenous promoter and human B7-H4 coding sequence was inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine B7h4 gene, resulting in a non-functional transcript.
      Protein expression analysis

      B7-H4 expression analysis in B-hB7-H4 B16-F10 cells by flow cytometry. Single cell suspensions from wild-type B16-F10 and B-hB7-H4 B16-F10 cultures were stained with species-specific anti-B7-H4 antibody. Human B7-H4 was detected on the surface of B-hB7-H4 B16-F10 cells, but not on the surface of wild-type B16-F10 cells. The 2-D09 clone of B-hB7-H4 B16-F10 cells was used for in vivo experiments.

      Protein expression analysis of tumor cells
      B-hB7-H4 B16-F10 cells were subcutaneously transplanted into C57BL/6 mice (n=5). At the end of the experiment, tumor cells were harvested and assessed for human B7-H4 expression by flow cytometry. As shown, human B7-H4 was highly expressed on the surface of tumor cells. Therefore, B-hB7-H4 B16-F10 cells can be used for in vivo efficacy studies of novel B7-H4 therapeutics.
      Tumor growth curve & Body weight changes

      Subcutaneous homograft tumor growth of B-hB7-H4 B16-F10 cells. Wild-type B16-F10 and B-hB7-H4 B16-F10 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H4 B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies.

      Subcutaneous homograft tumor growth of B-hB7-H4 B16-F10 cells. B-hB7-H4 B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hB7-H4 B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.