• 321926
| Product name | B-hEFEMP1 MC38 |
|---|---|
| Catalog number | 321926 |
| Strain background | C57BL/6 |
| Aliases | HDHRD; DRAD; FBNL; MLVT; MTLV; S1-5;FBLN3; FIBL-3 |
| Tissue | Colon |
| Disease | Colon carcinoma |
| Species | Mouse |
| Application | B-hEFEMP1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
DescriptionThe mouse Efemp1 gene was replaced by human EFEMP1 coding sequence in B-hEFEMP1 MC38 cells. Human EFEMP1 is highly expressed on the supernatant of B-hEFEMP1 MC38 cells.
Tageting strategy
Protein expression analysis
EFEMP1 expression analysis in B-hEFEMP1 MC38 cells by western blot. Human EFEMP1 was detected in the supernatant of B-hEFEMP1 MC38 cells but not wild-type MC38 cells. The 2-F12 clone of B-hEFEMP1 MC38 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hEFEMP1 MC38 cells. B-hEFEMP1 MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hEFEMP1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Protein expression analysis
