• 322002
| Product name | B-hGPNMB MC38 |
|---|---|
| Catalog number | 322002 |
| Strain background | C57BL/6 |
| Aliases | NMB; HGFIN; PLCA3 |
| Tissue | Colon |
| Disease | Colon carcinoma |
| Species | Mouse |
| Application | B-hGPNMB MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
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DescriptionThe mouse Gpnmb gene was replaced by human GPNMB coding sequence in B-hGPNMB MC38 cells. Human GPNMB is highly expressed on the surface of B-hGPNMB MC38 cells.
Tageting strategyGene targeting strategy for B-hGPNMB MC38 cells. The exogenous promoter and human GPNMB coding sequence was inserted to replace part of murine exons 2-3. The insertion disrupts the endogenous murine Gpnmb gene, resulting in a non-functional transcript.
Protein expression analysis
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hGPNMB MC38 cells. B-hGPNMB MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hGPNMB MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve of individual mice
B-hGPNMB MC38 tumor cells growth of individual mice. B-hGPNMB MC38 cells (5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6 mice (female, 6-week-old, n=6). As shown in panel, B-hGPNMB MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
