• 310815
| Product name | B-hLILRB4 MC38 |
|---|---|
| Catalog number | 310815 |
| Strain background | C57BL/6 |
| Aliases | ILT3, LIR5, CD85K, ILT-3, LIR-5 |
| Tissue | Colon |
| Disease | Colon carcinoma |
| Species | Mouse |
| Application | B-hLILRB4 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
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DescriptionThe human LILRB4 is expressed on the surface of B-hLILRB4 MC38 cells.
Targeting strategyGene targeting strategy for B-hLILRB4 MC38 cells. The exogenous promoter and human LILRB4 coding sequence were inserted to murine rosa26 site.
Protein expression analysis
LILRB4 expression analysis in B-hLILRB4 MC38 cells by flow cytometry. Single cell suspensions from B-hLILRB4 MC38 cultures were stained with species-specific anti-human LILRB4 antibody. Human LILRB4 was detected on the surface of B-hLILRB4 MC38 cells. The 5-C01 clone of B-hLILRB4 MC38 cells was used for in vivo experiments.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5×long diameter × short diameter2. As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells (5x105, 1x106, 5x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into B-hCD3E mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Subcutaneous homograft tumor growth of B-hLILRB4 MC38 cells. B-hLILRB4 MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hLILRB4 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
