C57BL/6 • 310807
| Product name | B-hROR1 MC38 |
|---|---|
| Catalog number | 310807 |
| Strain name | C57BL/6 |
| Strain background | C57BL/6 |
| Aliases | NTRKR1, dJ537F10.1 |
| Tissue | Colon |
| Disease | Colon carcinoma |
| Species | Mouse |
| Application | B-hROR1 MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
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DescriptionThe mouse Ror1 gene was replaced by human ROR1 coding sequence in B-hROR1 MC38 cells. Human ROR1 is highly expressed on the surface of B-hROR1 MC38 cells.
Targeting strategyGene targeting strategy for B-hROR1 MC38 cells. The exogenous promoter and human ROR1 coding sequence were inserted to replace part of murine exon 3 and all of exon 4. The insertion disrupts the endogenous murine Ror1 gene, resulting in a non-functional transcript.
Protein expression analysis
ROR1 expression analysis in B-hROR1 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hROR1 MC38 cultures were stained with species-specific anti-ROR1 antibody. Human ROR1 was detected on the surface of B-hROR1 MC38 cells, but not on the surface of wild-type MC38 cells. The 1-G06 clone of B-hROR1 MC38 cells was used for in vivo experiments.
Protein expression analysis of tumor cells
Subcutaneous homograft tumor growth of B-hROR1 MC38 cells. B-hROR1 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hROR1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hROR1 MC38 cells. B-hROR1 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=8). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hROR1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve of individual mice
B-hROR1 MC38 tumor growth of individual mice. B-hROR1 MC38 cells (5x105) and wild-type MC38 cells (5x105) were subcutaneously implanted into C57BL/6N mice (female, 8-week-old, n=8). As shown in panel, B-hROR1 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
