The exogenous promoter and mouse Car12 coding sequence were inserted into the mouse genome randomly. Mouse Car12 is highly expressed on the surface of B-Tg(mCar12) MC38 cells.
 

Gene targeting strategy for B-Tg(mCar12) MC38 cells. The exogenous promoter and mouse Car12 coding sequence were inserted into the mouse genome randomly.

Subcutaneous homograft tumor growth of B-Tg(mCar12) MC38 cells. B-Tg(mCar12) MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Tg(mCar12) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

B-Tg(mCar12) MC38 tumor growth of individual mice. B-Tg(mCar12) MC38 cells (5x10⁵) and wild-type MC38 cells (5x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old). As shown in panel, B-Tg(mCar12) MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.