B-luc B16-F10

C57BL/6 • 310680

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B-Luc A20
B-Luc CT26.WT

B-luc B16-F10

Product nameB-luc B16-F10
Catalog number310680
Strain nameC57BL/6
Strain backgroundC57BL/6
TissueSkin
DiseaseMelanoma
SpeciesMouse
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  • Description
  • Targeting strategy
  • Phenotypic analysis
  • Tumorigenicity

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      Description

      This B-luc B16-F10 cell line expresses firefly luciferase as a marker of B16-F10 cells. Luminescence can be observed B-luc B16-F10 cells.

      Targeting strategy

      Gene targeting strategy for B-luc B16-F10. The luciferase cDNA sequence with CAG promoter was inserted in the ROSA26 exon1 of wild-type B16-F10.

      In vitro luciferase activity of B-luc B16-F10 cells

      Luminescence signal intensity of B-luc B16-F10 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay. B-luc B16-F10 cells have a strong luminescence signal. The C5 clone of B-luc B16-F10 cells can be used in vivo experiments.

      Tumor growth curve & Body weight changes

      Subcutaneous homograft tumor growth of B-luc B16-F10 cells. B-luc B16-F10 cells and wild-type B16-F10 cells were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-luc B16-F10 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.

      Tumor growth and in vivo imaging of B-luc B16-F10 cells. B-luc B16-F10 cells (1x105 and 2x105) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-luc B16-F10 cells can be used for in vivo efficacy evaluation.