This B-Luc-EGFP HL-60 cell line expresses firefly luciferase as a marker of HL-60 cells. Fluorescence could be detected in B-Luc-EGFP HL-60 cells.

Gene targeting strategy for B-Luc-EGFP HL-60 cells. The CMV promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.

Luminescence signal intensity of B-Luc-EGFP HL-60 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay. B-Luc-EGFP HL-60 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP HL-60 cells. The 1-B06 clone of B-Luc-EGFP HL-60 cells can be used in vivo experiments.

B-Luc-EGFP HL-60 cells (1x10⁵) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP HL-60 cells can be used for in vivo efficacy evaluation.