This B-Luc LLC1 cell line expresses firefly luciferase as a marker of LLC1 cells. Luminescence can be observed in B-Luc LLC1 cells.

Gene targeting strategy for B-Luc LLC1 cells. The exogenous promoter and luciferase was inserted into the ROSA26 locus in murine.

Luminescence signal intensity of B-Luc LLC1 cells. Luminescence intensity was measured using the Bright-Glo^TM luciferase Assay. B-Luc LLC1 cells have a strong luminescence signal. The 1-H04 clone of B-Luc LLC1 cells can be used in vivo experiments.

Subcutaneous homograft tumor growth of B-Luc LLC1 cells. B-Luc LLC1 cells (1x10⁵ and 2x10⁵) and wild-type LLC1 cells (1x10⁵) were subcutaneously implanted into C57BL/6 mice (female, 9-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter². As shown in panel A, B-Luc LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.

Tumor growth and in vivo imaging of B-Luc LLC1 cells. B-Luc LLC1 cells (1x10⁵ and 2x10⁵) were subcutaneously implanted into the wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc LLC1 cells can be used for in vivo efficacy evaluation.

In vivo luciferase images of B-Luc LLC1 (C57BL/6, B-Luc LLC1, 1x10⁵, s.c.)