Protein expression analysis
Strain specific ADAM15 expression analysis in C57BL/6 mice and homozygous B-hADAM15 mice by western blot. Various tissue lysates were collected from wild-type C57BL/6 mice(+/+) and homozygous B-hADAM15 mice(H/H), and then analyzed by western blot with anti-ADAM15 antibody. 40 μg total proteins were loaded for western blotting analysis.
mRNA expression analysis
Strain specific analysis of ADAM15 mRNA expression in wild-type C57BL/6 mice and B-hADAM15 mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hADAM15 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ADAM15 primers. Mouse Adam15 mRNA was detectable only in wild-type C57BL/6 mice. Human ADAM15 mRNA was detectable only in homozygous B-hADAM15 mice but not in wild-type mice.
