Description
- CAXII (carbonic anhydrase 12) is a type I membrane enzyme highly expressed in colon, kidney.
- The exons 1~10 of mouse Car12 gene that encode signal peptide and extracellular domain are replaced by human counterparts in B-hCAXII mice. The genomic region of mouse Car12 gene that encodes transmembrane domain and cytoplasmic portion is retained. The chimeric CAXII expression is driven by endogenous mouse Car12 promoter.
- CAXII protein was detected in kidney, colon, stomach (weak expression), and skin (weak expression) of homozygous B-hCAXII mice.
- Application: This product is used for pharmacodynamics and safety evaluation of drugs.
mRNA expression analysis
Species specific analysis of CAXII gene expression in wild-type C57BL/6JNifdc mice and homozygous humanized B-hCAXII mice by RT-PCR. Kidney was collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCAXII mice (H/H). Mouse Car12 mRNA was detectable only in wild-type C57BL/6JNifdc mice. Human CAXII mRNA was detectable only in homozygous B-hCAXII mice, but not in wild-type C57BL/6JNifdc mice.
Western blot analysis of CAXII protein expression in homozygous B-hCAXII mice. Various tissue lysates were collected from wild-type C57BL/6JNifdc mice (+/+) and homozygous B-hCAXII mice (H/H), and then analyzed by western blot with species-specific anti-CAXII antibody (CST, 5864). 40 μg total proteins were loaded for western blotting analysis. The high expression of CAXII protein was detected in kidney and colon of homozygous B-hCAXII mice compared to the stomach and skin.
