Gene targeting strategy for B-hCCR2 mice. The exons 3 of mouse Ccr2 gene that encode the full-length protein domain was replaced by human CCR2 exons 2-3 in B-hCCR2 mice.

Strain specific CCR2 expression analysis in homozygous B-hCCR2 mice by flow cytometry. Bone marrow was collected from WT and homozygous B-hCCR2 mice, and analyzed by flow cytometry with species-specific anti-CCR2 antibody. Mouse CCR2 was only detectable in WT mice. Human CCR2 was exclusively detectable in homozygous B-hCCR2 but not WT mice.