Gene targeting strategy for B-hCD105 mice.
The exons 2~14 of mouse Eng gene that encode the extracellular domain were replaced by human ENG exons 2~14 in B-hCD105 mice.

Western blot analysis of CD105 protein expression in homozygous B-hCD105 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD105 mice (H/H), and then analyzed by western blot with species-specific anti-CD105 antibody. 20 μg total proteins were loaded for western blotting analysis. hCD105 was detected in lung,heart,spleen,kidney,liver and skin from homozygous mice.

Strain specific analysis of CD105 mRNA expression in wild-type C57BL/6 mice and B-hCD105 mice by RT-PCR. Lung RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hCD105 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CD105 primers. Mouse Cd105 mRNA was detectable only in wild-type C57BL/6 mice. Human CD105 mRNA was detectable only in homozygous B-hCD105 mice but not in wild-type mice.