C57BL/6-Cd3etm2(CD3E)Bcgen Tnfrsf9tm1(TNFRSF9)Bcgen/Bcgen • 121281
| Product name | B-hCD3E/h4-1BB mice |
|---|---|
| Catalog number | 121281 |
| Strain name | C57BL/6-Cd3etm2(CD3E)Bcgen Tnfrsf9tm1(TNFRSF9)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 12501,21942 |
| Aliases | CD3E,CD3e molecule, IMD18, T3E, TCRE; TNFRSF9, TNF receptor superfamily member 9,4-1BB, CD137, CDw137, ILA |
Targeting strategyGene targeting strategy for B-hCD3E/h4-1BB mice.
The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/h4-1BB mice. The exons 2-7 of mouse Tnfrsf9 gene that encode the extracellular domain were replaced by human TNFRSF9 exons 3-8 in B-hCD3E/h4-1BB mice.
Protein expression analysis
Strain specific CD3E expression analysis in homozygous B-hCD3E/h4-1BB mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/h4-1BB mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/h4-1BB mice (H/H;H/H) but not in WT mice (+/+) .
Strain specific 4-1BB expression analysis in homozygous B-hCD3E/h4-1BB mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/h4-1BB mice (H/H;H/H) stimulated with anti-CD3ε antibody in vivo, and analyzed by flow cytometry with species-specific anti-CD3E antibody. Mouse 4-1BB was detectable in WT mice (+/+). Human 4-1BB was exclusively detectable in homozygous B-hCD3E/h4-1BB mice (H/H;H/H) but not in WT mice (+/+) .
