Gene targeting strategy for B-hCD79B mice. The exons 1~4 of mouse Cd79b gene that encode the extracellular domain were replaced by human CD79B exons 1~4 in B-hCD79B mice.

Strain specific CD79B expression analysis in heterozygous B-hCD79B mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD79B (H/+) mice, and analyzed by flow cytometry with species-specific CD79B antibody. Mouse CD79B was detectable in WT mice and heterozygous B-hCD79B. Human CD79B was exclusively detectable in heterozygous B-hCD79B but not WT mice.