C57BL/6N-Cx3cl1tm1(CX3CL1)Bcgen/Bcgen • 111257
| Product name | B-hCX3CL1 mice |
|---|---|
| Catalog number | 111257 |
| Strain name | C57BL/6N-Cx3cl1tm1(CX3CL1)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| Aliases | CX3CL1 (ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin) |
Targeting strategyGene targeting strategy for B-hCX3CL1 mice. The exons 2-3 of mouse Cx3cl1 gene that encode the extracellular domain were replaced by human CX3CL1 exons 2-3 in B-hCX3CL1 mice.
mRNA expression analysis
Strain specific analysis of CX3CL1 gene expression in wild-type C57BL/6 mice and B-hCX3CL1 mice by RT-PCR. Mouse Cx3cl1 mRNA was detectable in brain of wild-type C57BL/6 mice (+/+). Human CX3CL1 mRNA was detectable only in homozygous B-hCX3CL1 mice (H/H) but not in wild-type mice.
Protein expression analysis
Strain specific CX3CL1 expression analysis in homozygous B-hCX3CL1 mice by ELISA. The brain homogenates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCX3CL1 mice (H/H), and analyzed by ELISA with CX3CL1 ELISA kit. Mouse CX3CL1 was detectable in wild-type mice. Human CX3CL1 was detectable in homozygous B-hCX3CL1 mice, these two ELISA kits cross-identify human and mouse CX3CL1 protein.
