C57BL/6N-Cxcl13tm1(CXCL13)Bcgen/Bcgen • 111136
| Product name | B-hCXCL13 mice |
|---|---|
| Catalog number | 111136 |
| Strain name | C57BL/6N-Cxcl13tm1(CXCL13)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| NCBI gene ID | 55985 |
| Aliases | Chemokine (C-X-C motif) ligand 13 (CXCL13),B lymphocyte chemoattractant (BLC) or B cell-attracting chemokine 1 (BCA-1) |
Targeting strategyGene targeting strategy for B-hCXCL13 mice. The exons 1~4 of mouse Cxcl13 gene that encode the full-length protein were replaced by human CXCL13 exons 1~4 in B-hCXCL13 mice.
mRNA expression analysis
Strain specific analysis of CXCL13 gene expression in wild-type C57BL/6 mice and homozygous B-hCXCL13 mice by RT-PCR. Mouse Cxcl13 mRNA was detectable in splenocytes of wild-type C57BL/6 mice (+/+). Human CXCL13 mRNA was detectable only in homozygous B-hCXCL13 mice, but not in wild-type mice.
Protein expression analysis
Strain specific CXCL13 expression analysis in homozygous B-hCXCL13 mice by ELISA. Serum were collected from wild-type C57BL/6 mice and homozygous B-hCXCL13 mice (H/H), and analyzed by ELISA with species-specific CXCL13 ELISA kit. Mouse CXCL13 was only detectable in wild-type mice. Human CXCL13 was exclusively detectable in homozygous B-hCXCL13 mice but not in wild-type mice.
