Gene targeting strategy for B-hFGFRL1 mice. The exons 3-7 of mouse Fgfrl1 gene that encode the extracellular domain were replaced by human FGFRL1 exons 3-7 in B-hFGFRL1 mice.

Strain specific analysis of FGFRL1 mRNA expression in wild-type C57BL/6 mice and B-hFGFRL1 mice by RT-PCR. Kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hFGFRL1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FGFRL1 primers. Mouse Fgfrl1 mRNA was detectable only in wild-type C57BL/6 mice. Human FGFRL1 mRNA was detectable only in homozygous B-hFGFRL1 mice but not in wild-type mice.