Experimental results
Experimental results Splenocytes-T cells
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in T cells of wild-type mice, while hITGA2 was not detectable in T cells of homozygous mice.
Experimental results Splenocytes-B cells
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in B cells of wild-type mice, while hITGA2 was not detectable in B cells of homozygous mice.
Experimental results Splenocytes-NK cells
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in NK cells of wild-type mice, while hITGA2 was detectable in NK cells of homozygous mice.
Experimental results Splenocytes-monocytes
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes of wild-type mice, while hITGA2 was detectable in monocytes of homozygous mice.
Experimental results Splenocytes-macrophages
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in macrophages of wild-type mice, while hITGA2 was not detectable in macrophages of homozygous mice.
Protein expression profile in splenocytes
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes and NK cells of wild-type mice, while hITGA2 was detectable in monocytes and NK cells of homozygous mice.
Experimental results Bone marrow cells-B cells
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in B cells of wild-type mice, while hITGA2 was not detectable in B cells of homozygous mice.
Experimental results Bone marrow cells-NK cells
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in NK cells of wild-type mice, while hITGA2 was detectable in NK cells of homozygous mice.
Experimental results Bone marrow cells-monocytes
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes of wild-type mice, while hITGA2 was detectable in monocytes of homozygous mice.
Experimental results Bone marrow cells-macrophages
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was not detectable in macrophages of wild-type mice, while hITGA2 was not detectable in macrophages of homozygous mice.
Protein expression profile in bone marrow cells
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in monocytes and NK cells of wild-type mice, while hITGA2 was detectable in monocytes and NK cells of homozygous mice.
Experimental results
Experimental results Platelets
Strain specific ITGA2 expression analysis in homozygous B-hITGA2 mice by flow cytometry. Platelets were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA2 mice (H/H), and analyzed by flow cytometry with species-specific anti-ITGA2 antibody. mITGA2 was detectable in platelets of wild-type mice, while hITGA2 was detected in platelets of homozygous mice.
Targeting strategy
