Western blot analysis of ITGA9 protein expression in homozygous B-hITGA9 mice. Various tissue lysates were collected from from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA9 mice (H/H) , and then analyzed by western blot with anti-ITGA9 antibody. 40 μg total proteins were loaded for western blotting analysis.

Strain specific analysis of ITGA9 mRNA expression in wild-type C57BL/6 mice and B-hITGA9 mice by RT-PCR. Lung RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hITGA9 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human ITGA9 primers. Mouse Itga9 mRNA was detectable only in wild-type C57BL/6 mice. Human ITGA9 mRNA was detectable only in homozygous B-hITGA9 mice but not in wild-type mice.