C57BL/6-Pdcd1tm1(PDCD1)BcgenCd274tm1(CD274)BcgenKdrtm1(KDR)Bcgen/Bcgen • 112494
| Product name | B-hPD-1/hPD-L1/hVEGFR2 mice |
|---|---|
| Catalog number | 112494 |
| Strain name | C57BL/6-Pdcd1tm1(PDCD1)BcgenCd274tm1(CD274)BcgenKdrtm1(KDR)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| Aliases | PD-1(CD279, PDCD1, PD1, SLEB2, hPD-1, hPD-l, hSLE1)； PD-L1 (B7-H, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1, hPD-L1) VEGFR2（CD309, FLK1, VEGFR, KDR） |
Protein expression analysis
Strain specific PD-1 expression analysis in wild-type C57BL/6 and homozygous B-hPD-1/hPD-L1/hVEGFR2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hVEGFR2 mice (H/H) after stimulated with anti-CD3ε in vivo or not. Mouse PD-1 was detectable on CD4+ T cells and CD8+ T cell in wild-type C57BL/6 mice. Human PD-1 was exclusively detectable on CD4+ T cells and CD8+ T cell in homozygous B-hPD-1/hPD-L1/hVEGFR2 mice but not in wild-type mice.
Strain specific PD-L1 expression analysis in wild-type C57BL/6 and homozygous B-hPD-1/hPD-L1/hVEGFR2 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hVEGFR2 mice (H/H) after stimulated with anti-CD3ε in vivo or not. Mouse PD-L1 was detectable on CD4+ T cells and CD8+ T cell in wild-type C57BL/6 mice. Human PD-L1 was exclusively detectable on CD4+ T cells and CD8+ T cell in homozygous B-hPD-1/hPD-L1/hVEGFR2 mice but not in wild-type mice.
Strain specific VEGFR2 expression analysis in wild-type C57BL/6 and homozygous B-hPD-1/hPD-L1/hVEGFR2 mice by flow cytometry. Lung endothelial cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1/hPD-L1/hVEGFR2 mice (H/H). Mouse VEGFR2 was detectable in lung endothelial cells in wild-type C57BL/6 mice. Human VEGFR2 was only detectable in homozygous B-hPD-1/hPD-L1/hVEGFR2 mice but not in wild-type mice.
