C57BL/6-Pdcd1tm3(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Pdcd1lg2tm3(PDCD1LG2)Bcgen/Bcgen • 112710
| Product name | B-hPD-1 plus/hPD-L1/hPD-L2 mice |
|---|---|
| Catalog number | 112710 |
| Strain name | C57BL/6-Pdcd1tm3(PDCD1)Bcgen Cd274tm1(CD274)Bcgen Pdcd1lg2tm3(PDCD1LG2)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 5133, 29126, 80380 |
| Aliases | CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1;B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1;B7DC, Btdc, CD273, PD-L2, PDCD1L2, PDL2, bA574F11.2 |
mRNA expression analysis in humanized B-hPD-1 plus/hPD-L1/hPD-L2 mice
Species specific analysis of PD-L2 gene expression in wild-type C57BL/6 mice and homozygous humanized B-hPD-1 plus/hPD-L1/hPD-L2 mice by RT-PCR. Thymus was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice (H/H). Mouse PD-L2 mRNA was detectable only in wild-type C57BL/6 mice. Human PD-L2 mRNA was only detectable in homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice, but not in wild-type C57BL/6 mice.
Protein expression analysis in spleen
Strain specific PD-L2 expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hPD-1 plus/hPD-L1/hPD-L2 mice by flow cytometry. Bone marrow was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice (H/H) and cultured in vitro to induce the bone marrow-derived dendritic cells (BMDCs). The successfully induced BMDCs were stimulated with or without LPS in vitro. Expression of PD-L2 on BMDCs was analyzed by flow cytometry. Mouse PD-L2 was only detectable in wild-type C57BL/6 mice. Human PD-L2 was exclusively detectable in homozygous B-hPD-1 plus/hPD-L1/hPD-L2 mice, but not in wild-type C57BL/6 mice.
