B-hSEMA4D mice

C57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen • 112334

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B-hSEMA4D mice

Product nameB-hSEMA4D mice
Catalog number112334
Strain nameC57BL/6-Sema4dtm1(SEMA4D)Bcgen/Bcgen
Strain backgroundC57BL/6
AliasesSEMA4D (A8, GR3, BB18, CD100, COLL4, SEMAJ, coll-4, C9orf164, M-sema-G)
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hSEMA4D mice. The chimeric SEMA4D CDS (replace the extracellular domain) was inserted in the exon 4 of mouse Sema4d gene in B-hSEMA4D mice.

      Protein expression analysis (T cells)

      Strain specific SEMA4D expression analysis in T cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

      Protein expression analysis (B cells)

      Strain specific SEMA4D expression analysis in B cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.

      Protein expression analysis (NK cells)
      Strain specific SEMA4D expression analysis in NK cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (DC cells)
      Strain specific SEMA4D expression analysis in DC cells of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (granulocytes)
      Strain specific SEMA4D expression analysis in granulocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (macrophages)
      Strain specific SEMA4D expression analysis in macrophages of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.
      Protein expression analysis (monocytes)

      Strain specific SEMA4D expression analysis in monocytes of homozygous B-hSEMA4D mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hSEMA4D mice (H/H), and analyzed by flow cytometry with species-specific anti-SEMA4D antibody. Mouse SEMA4D was detectable in wild-type mice. Human SEMA4D was exclusively detectable in homozygous B-hSEMA4D but not in wild-type mice.