C57BL/6N-Slc16a3tm1(SLC16A3)Bcgen/Bcgen • 112591
| Product name | B-hSLC16A3 mice |
|---|---|
| Catalog number | 112591 |
| Strain name | C57BL/6N-Slc16a3tm1(SLC16A3)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| Aliases | SLC16A3: MCT3, MCT4, MCT 3, MCT 4, MCT-3, MCT-4 |
Targeting strategyGene targeting strategy for B-hSLC16A3 mice. The exons 2-4 of mouse Slc16a3 gene that encode the full-length protein were replaced by human SLC16A3 exons 2-5 in B-hSLC16A3 mice.
Protein expression analysis
mRNA expression analysis
Strain specific analysis of SLC16A3 mRNA expression in wild-type C57BL/6N mice and B-hSLC16A3 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hSLC16A3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SLC16A3 primers. Mouse Slc16a3 mRNA was detectable only in wild-type C57BL/6N mice. Human SLC16A3 mRNA was detectable only in homozygous B-hSLC16A3 mice but not in wild-type mice.
