• Somatostatin Receptor 2 (SSTR2) is one of five 7-transmembrane G-protein-coupled receptors for somatostatins 14 and 28. Human SSTR2 shares 84% aa identity with mouse SSTR2 within the extracellular domains. Isoform B (357 aa) has an alternate C-terminal cytoplasmic region that is 12 aa shorter than that of isoform A (369 aa, reported as 93 kDa). Both are expressed in brain, stomach, intestinal epithelia, pancreatic islets and kidney tubules. Isoform B is also expressed in parotid, thyroid and bronchial glands.
• The exon 2 of mouse Sstr2 gene that encode the extracellular and transmembrane domains was replaced by human SSTR2 exon 2 in B-hSSTR2 mice.
• Mouse Sstr2 mRNA was detectable in cerebellum of wild-type C57BL/6 mice. Human SSTR2 mRNA was detectable in homozygous B-hSSTR2 mice.
• Human SSTR2 was detectable in cerebellum, pancreas, stomach, esophagus and uterus of homozygous B-hSSTR2 mice.
• Application: This product is used for the efficacy evaluation and safety evaluation of drugs for the treatment of neuroendocrine tumors.

Strain specific analysis of SSTR2 mRNA expression in wild-type C57BL/6 mice and B-hSSTR2 mice by RT-PCR. Cerebellum RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hSSTR2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SSTR2 primers. Mouse Sstr2 mRNA was only detectable in wild-type C57BL/6 mice. Human SSTR2 mRNA was only detectable in homozygous B-hSSTR2 mice.

Western blot analysis of SSTR2 protein expression in homozygous B-hSSTR2 mice. Various tissue lysates were collected from wild-type C57BL/6N (+/+) mice and homozygous B-hSSTR2 mice (H/H), and then analyzed by western blot with anti-SSTR2 antibody. 40 μg total proteins were loaded for western blotting analysis. SSTR2 was detected in cerebellum, pancreas, stomach, esophagus and uterus.