B-hTNFR2/hTNFR1/hLTBR mice

C57BL/6-Tnfrsf1btm1(TNFRSF1B)Bcgen Tnfrsf1atm1(TNFRSF1A)Bcgen Ltbrtm1(LTBR)Bcgen/Bcgen • 140801

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B-hTNFR2/hTNFR1 mice
B-hTNFRSF11A mice

B-hTNFR2/hTNFR1/hLTBR mice

Product nameB-hTNFR2/hTNFR1/hLTBR mice
Catalog number140801
Strain nameC57BL/6-Tnfrsf1btm1(TNFRSF1B)Bcgen Tnfrsf1atm1(TNFRSF1A)Bcgen Ltbrtm1(LTBR)Bcgen/Bcgen
Strain backgroundC57BL/6
AliasesTNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR, TNF receptor superfamily member 1B; TNFRSF1A, CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60, TNF receptor superfamily member 1A; LTBR, D12S370, LT-BETA-R, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFR3, TNFRSF3, lymphotoxin beta receptor
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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hTNFR2/hTNFR1/hLTBR mice. The exons 2~6 of mouse Tnfr2 gene that encode the extracellular domain were replaced by human TNFR2 exons 2~6 in B-hTNFR2/hTNFR1/hLTBR mice. The exons 2~6 of mouse Tnfr1 gene that encode the extracellular domain were replaced by human TNFR1 exons 2~6 in B-hTNFR2/hTNFR1/hLTBR mice. The exons 1-7 of mouse Ltbr gene that encode the extracellular domain were replaced by human LTBR exons 1-7 in B-hTNFR2/hTNFR1/hLTBR mice.

      Protein expression analysis in granulocytes
      Strain specific TNFR1 expression analysis in homozygous B-hTNFR2/hTNFR1/hLTBR mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hTNFR2/hTNFR1/hLTBR mice (H/H;H/H;H/H), and analyzed by flow cytometry with species-specific anti-TNFR1 antibody. Mouse TNFR1 was detectable in WT mice (+/+). Human TNFR1 was exclusively detectable in homozygous B-hTNFR2/hTNFR1/hLTBR mice (H/H;H/H;H/H) but not in WT mice (+/+).
      Protein expression analysis in macrophages
      Strain specific LTBR expression analysis in homozygous B-hTNFR2/hTNFR1/hLTBR mice by flow cytometry.Spleen, blood and bone marrow were collected from wild type (WT) mice (+/+) and homozygous B-hTNFR2/hTNFR1/hLTBR mice (H/H; H/H; H/H), and analyzed by flow cytometry with species-specific anti-LTBR antibody. Mouse LTBR was detectable in WT mice (+/+). Human LTBR was exclusively detectable in homozygous B-hTNFR2/hTNFR1/hLTBR mice (H/H; H/H; H/H) but not in WT mice (+/+).
      Protein expression analysis in regulatory T cells (Tregs)

      Strain specific TNFR2 expression analysis in homozygous B-hTNFR2/hTNFR1/hLTBR mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hTNFR2/hTNFR1/hLTBR mice (H/H;H/H;H/H) stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-TNFR2 antibody. Mouse TNFR2 was detectable in WT mice (+/+). Human TNFR2 was exclusively detectable in homozygous B-hTNFR2/hTNFR1/hLTBR mice (H/H;H/H;H/H) but not in WT mice (+/+).