• TNFRSF11B is a bait receptor secreted by osteoblasts, which binds to RANKL and neutralizes the conduction of RANKL and RANK in the osteoclast signaling pathway.
• B-hTNFRSF11B mice used for myeloma and osteoporosis.

Gene targeting strategy for B-hTNFRSF11B mice. Exon 2~5 of the mouse Tnfrsf11b gene that encodes the full-length protein was replaced by human TNFRSF11B exon 2~5 in B-hTNFRSF11B mice.

Strain specific TNFRSF11B expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hTNFRSF11B mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTNFRSF11B mice (H/H) (n=3, 6-week-old). Protein expression level of TNFRSF11B was analyzed by ELISA. Mouse TNFRSF11B was only detectable in wild-type C57BL/6 mice. Human TNFRSF11B was exclusively detectable in homozygous B-hTNFRSF11B mice.