• TRPM4 is a calcium-activated non selective cation channel which mediated cell membrane depolarization.
• Homozygous mice expressed chimeric TRPM4 and developed normally.
• This model is useful in studying the role of TRPM4 in cardiomyocytes, neurons and cochlea hair cells.

Gene targeting strategy for B-hTRPM4 mice. The exons 17-20 of mouse Trpm4 gene that encode transmembrane domain, extracellular domain and part of cytoplasmic region is replaced by human counterparts in B-hTRPM4 mice. The genomic region of mouse Trpm4 gene that encodes N- and C- terminal cytoplasmic portion is retained. The promoter, 5’UTR and 3’UTR region of the mouse gene are also retained. The chimeric TRPM4 expression is driven by endogenous mouse Trpm4 promoter, while mouse Trpm4 gene transcription and translation will be disrupted.

Western blot analysis of TRPM4 protein expression in homozygous B-hTRPM4 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hTRPM4 mice (H/H), and then analyzed by western blot with anti-TRPM4 antibody (Abcam, ab106200). 40 μg total proteins were loaded for western blotting analysis. TRPM4 was detectable in brain, heart (at a low level), lung, liver, large intestine and stomach.