Protein expression analysis
Strain specific analysis of FcRn expression in B-NDG mice and B-NDG hFcRn mice by western blot. Liver, kidney and spleen were collected from B-NDG mice (+/+) and homozygous B-NDG hFcRn mice (H/H) and analyzed by western blot with species-specific anti-FcRn antibody. Mouse FcRn was detectable in B-NDG mice (+/+). Human FcRn was detectable only in homozygous B-NDG hFcRn mice (H/H) but not in B-NDG mice (+/+).
Serum half-life of antibody drug in B-NDG hFcRn mice
Pharmacokinetics of YERVOY-analog (isotype: human IgG1) in B-NDG hFcRn mice.B-NDG mice and B-NDG hFcRn mice were intravenously injected with YERVOY (in house), the isotype of YERVOY (in house) is human IgG1, and serum was collected for pharmacokinetic (PK) analysis (n=3). A, The PK curve. B, The PK parameters. Data are shown in mean ± SEM.
