Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6, NOD-scid, B-NDG mice, B-NDG nude mice (n=5, 6-week-old, female ). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for mCD45 population and used for further analysis as indicated here. B. Results of FACS analysis. T cells, B cells and NK cells were not detectable in both B-NDG mice and B-NDG nude mice.

B-NDG nude mice showed defects in hair development. (A) B-NDG nude mouse. B-NDG mouse were inoculated with human breast cancer MDA-MB-231 cells in the unilateral mammary pad, and B-NDG nude mice was easier to observe for subcutaneous tumor transplantation. Photograph of B-NDG nude mouse tumor formation (B); Photograph of B-NDG mouse tumor formation (C).