Description
Protein expression analysis
In vivo efficacy of anti-human PD-1 antibody
Protein expression analysis
Strain specific PD-1 expression analysis in homozygous B-hPD-1 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Mouse PD-1 was detected in WT mice. Human PD-1 was exclusively detected in homozygous B-hPD-1 mice but not WT mice.
In vivo efficacy of anti-human PD-1 antibody
Antitumor activity of anti-human PD-1 antibody in B-hPD-1 mice.(A) Anti-human PD-1 antibody inhibited MC38 tumor growth in B-hPD-1 mice. Murine colon cancer MC38 cells (5×105) were subcutaneously implanted into homozygous B-hPD-1 mice (male, 4-6 week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with anti-human PD-1 antibody with doses and schedules indicated in panel (A). (B) Body weight changes during treatment. As shown in panel A, anti-human PD-1 antibody was efficacious in controlling tumor growth in B-hPD-1 mice, demonstrating that B-hPD-1 mice provide a powerful preclinical model for in vivo evaluation of anti-human PD-1 antibodies. Values are expressed as mean ± SEM.
