C57BL/6-Cd70tm1(CD70)Bcgen/Bcgen • 110770
| Product name | B-hCD70 mice |
|---|---|
| Catalog number | 110770 |
| Strain name | C57BL/6-Cd70tm1(CD70)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 21948 |
| Aliases | CD70, CD70 molecule, CD27L, LPFS3, CD27-L, CD27LG, TNFSF7, TNLG8A |
Targeting strategyGene targeting strategy for B-hCD70 mice.
The exons 1-3 of mouse Cd70 gene that encode the extracellular domain were replaced by human CD70 exons 1-3 in B-hCD70 mice.
mRNA expression analysis
Strain specific analysis of CD70 gene expression in wild type (WT) mice and B-hCD70 mice by RT-PCR. Mouse CD70 mRNA was detectable only in thymocytes of WT mice (+/+) and B-hCD70 mice (H/+). Human CD70 mRNA was detectable only in homozygous B-hCD70 mice (H/+) but not in WT mice (+/+).
Protein expression analysis-Dendritic cells
Strain specific CD70 expression analysis in homozygous B-hCD70 mice by flow cytometry. Bone marrow cells were collected from wild type (WT) mice (+/+) and homozygous B-hCD70 mice (H/H) stimulated with LPS in vivo, and analyzed by flow cytometry with species-specific anti-CD70 antibody. Mouse CD70 was detectable in WT mice (+/+). Human CD70 was exclusively detectable in homozygous B-hCD70 mice (H/H) but not in WT mice (+/+).
