B-hEGFR/hMET mice

C57BL/6-Egfrtm2(EGFR)BcgenMettm1(MET)Bcgen/Bcgen • 112258

B-hEGFR/hHER3 mice
B-hEGFR/hMET/hHGF mice

B-hEGFR/hMET mice

Product nameB-hEGFR/hMET mice
Catalog number112258
Strain nameC57BL/6-Egfrtm2(EGFR)BcgenMettm1(MET)Bcgen/Bcgen
Strain backgroundC57BL/6
AliasesEGFR, ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor; MET, AUTS9, DFNB97, HGFR, RCCP2, c-Met, MET proto-oncogene, receptor tyrosine kinase

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  • Targeting strategy
  • Phenotypic analysis

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    Publication

      Targeting strategy

      Gene targeting strategy for B-hEGFR/hMET mice. The exons 2-17 of mouse Egfr gene that encode the extracellular domain were replaced by human EGFR exons 2-17 in B-hEGFR/hMET mice. The exons 3-14 of mouse Met gene that encode the extracellular domain were replaced by human MET exons 2-13 in B-hEGFR/hMET mice.

      mRNA expression analysis

      Strain specific analysis of EGFR gene expression in wild type (WT) mice and B-hEGFR/hMET mice by RT-PCR. Mouse Egfr mRNA was detectable only in liver and kidney of WT mice (EGFR+/+MET+/+). Human EGFR mRNA was detectable only in B-hEGFR/hMET mice (EGFRH/HMETH/+) but not in WT mice (EGFR+/+MET+/+). The positive band was confirmed to be correct by sequencing.

      Strain specific analysis of MET gene expression in wild type (WT) mice and B-hEGFR/hMET mice by RT-PCR. Mouse Met mRNA was detectable in liver and kidney of WT mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+). Human MET mRNA was detectable only in B-hEGFR/hMET mice (EGFRH/HMETH/+) but not in WT mice (EGFR+/+MET+/+). The positive band was confirmed to be correct by sequencing.

      Protein expression analysis

      Strain specific MET expression analysis in homozygous B-hEGFR/hMET mice by western blot. Liver and kidney were collected from wild type (WT) mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+), and analyzed by western blot with anti-MET antibody. MET was detectable in WT mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+) due to the cross-reactivity of antibodies.

      IHC analysis of EGFR expression

      Immunohistochemical (IHC) analysis of EGFR expression in homozygous B-hEGFR/hMET mice. The liver and kidney were collected from wild type (WT) mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+) and analyzed by IHC with anti-EGFR antibody. EGFR was detectable in WT mice (EGFR+/+MET+/+) and B-hEGFR/hMET mice (EGFRH/HMETH/+) due to the cross-reactivity of antibodies.