B-hBTLA/hHVEM mice

C57BL/6-Btlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen • 121080

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B-hBTLA mice
B-hBTN3A2/hBTN2A2/hBTN3A1/hBTN3A3/hBTN2A1 mice

B-hBTLA/hHVEM mice

Product nameB-hBTLA/hHVEM mice
Catalog number121080
Strain nameC57BL/6-Btlatm1(BTLA)Bcgen Tnfrsf14tm1(TNFRSF14)Bcgen/Bcgen
Strain backgroundC57BL/6
AliasesBTLA, BTLA1, CD272, B and T lymphocyte associated; TNFRSF14, HVEM, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, Herpesvirus entry mediator, TNF receptor superfamily member 14
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  • Phenotypic analysis
  • Efficacy

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      mRNA expression analysis
      Strain specific analysis of BTLA and HVEM gene expression in wild type (WT) mice and B-hBTLA/hHVEM mice by RT-PCR.Mouse Btla and Hvem mRNA was detectable only in splenocytes of WT mice (+/+). Human BTLA and HVEM mRNA was detectable only in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
      Protein expression analysis in B cells
      Strain specific BTLA expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA was detectable in WT mice (+/+). Human BTLA was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
      Protein expression analysis in T cells
      Strain specific HVEM expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-HVEM antibody. Mouse HVEM was detectable in WT mice (+/+). Human HVEM was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).
      Tumor growth curve & Body weight changes›

      Subcutaneous homograft tumor growth of B-hHVEM MC38 cells. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean ± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hHVEM MC38 cells were able to form tumors in vivo and can be used for efficacy studies.

      Tumor growth curve of individual mice

      B-hHVEM MC38 tumor cells growth of individual mice. B-hHVEM MC38 cells (1x106) and wild-type MC38 cells (5x105) were subcutaneously implanted into homozygous B-hBTLA/hHVEM mice (female, 6-week-old, n=7). As shown in panel, B-hHVEM MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.