C57BL/6-Ltatm1(LTA)Bcgen Tnftm1(TNF)Bcgen Ltbtm1(LTB)Bcgen/Bcgen • 130769
| Product name | B-hLTA/hTNFA/hLTB mice |
|---|---|
| Catalog number | 130769 |
| Strain name | C57BL/6-Ltatm1(LTA)Bcgen Tnftm1(TNF)Bcgen Ltbtm1(LTB)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 16992,21926,16994 |
| Aliases | LTA(lymphotoxin alpha); TNF(tumor necrosis factor); LTB(lymphotoxin beta) |
mRNA expression analysis
Strain specific analysis of LTA, TNFA and LTB gene expression in wild type (WT) mice and B-hLTA/hTNFA/hLTB mice by RT-PCR. Mouse Lta, Tnfa and Ltb mRNA were detectable only in splenocytes of WT mice (+/+). Human LTA, TNFA and LTB mRNA were detectable only in homozygous B-hLTA/hTNFA/hLTB mice (H/H) but not in WT mice (+/+).
Protein expression analysis
Strain specific LTA expression analysis in wild type (WT) mice and B-hLTA/hTNFA/hLTB mice by ELISA. Serum was collected from WT mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H) stimulated with LPS in vivo, and analyzed by ELISA with anti-LTA ELISA kit. LTA was detectable in WT mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H) due to the cross-reactivity of antibodies.
Strain specific TNFA expression analysis in wild type (WT) mice and B-hLTA/hTNFA/hLTB mice by ELISA. Serum was collected from WT mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H) stimulated with LPS in vivo, and analyzed by ELISA with species-specific TNFA ELISA kit. Mouse TNFA was detectable in WT mice. Human TNFA was exclusively detectable in homozygous B-hLTA/hTNFA/hLTB mice (H/H), but not in WT mice (+/+) . ND: not detectable.
Strain specific LTB expression analysis in wild type (WT) mice and B-hLTA/hTNFA/hLTB mice by western blot. Spleen and thymus were collected from WT mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H), and analyzed by western blot with anti-LTB antibody. LTB was detectable in WT mice (+/+) and homozygous B-hLTA/hTNFA/hLTB mice (H/H) due to the cross-reactivity of antibodies.
