Results

Orthotopic Breast Cancer Model: 4T1 in Balb/c Mice

Establishment of orthotopic breast cancer model.
After the inoculation of 4T1 cells in Balb/c mice (F, n=5), the orthotopic tumor can achieve a stable and moderate growth rate, and had no effect on body weight. Flow cytometry showed that there was a significant difference in the number of macrophages and DC cells between orthotopic tumor and subcutaneous tumor. H&E staining showed that tumors metastasized to the lung.

Orthotopic Breast Cancer Model: MDA-MB-231 in B-NDG Mice

Establishment of orthotopic breast cancer model.
MDA-MB-231 cells (1.5x10⁷and 2x10⁷) were mammary pad into B-NDG mice (female, 11-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, MDA-MB-231 cells were able to establish tumors in vivo and can be used for efficacy studies.

Orthotopic Breast Cancer Model: EO771 in C57BL/6N Mice

Establishment of orthotopic breast cancer model.
EO771 cells were subcutaneously (5x10⁴, 1x10⁵) and mammary pad (2x10⁴, 5x10⁴, , 1x10⁵ , 2x10⁵) into C57BL/6 mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, EO771 cells were able to establish tumors in vivo and can be used for efficacy studies.

Orthotopic Breast Cancer Model: DU4475 in B-NDG B2m KO Mice

Establishment of orthotopic breast cancer model.
DU4475 cells (2x10⁶, 5x10⁶, 1x10⁷) were subcutaneously and mammary pad into B-NDG B2m KO mice (female, 11-week-old). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm³ using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, DU4475 cells were able to establish tumors in vivo and can be used for efficacy studies.

Orthotopic Breast Cancer Model: BT-474 in B-NDG Mice

Establishment of orthotopic breast cancer model.
BT-474 cells (1x10⁷,2x10⁷) were subcutaneously and mammary pad into B-NDG mice (female, 8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 × long diameter × short diameter2. As shown in panel A, BT-474 cells were able to establish tumors in vivo and can be used for efficacy studies.