The expression of EGFP and tdTomato in mouse erythrocytes was detected by flow cytometry. The majority of erythrocytes in the bone marrow of B-Epor-EGFP- iCre(Mut/+); Rosa26-CAG- tdTomato(Mut/+) mice were double positive cells of EGFP and tdTomato, while the granulocytes in bone marrow were single positive cells of tdTomato. The results were consistent with the results in the study, indicating that B-Epor-EGFP-iCre mice were designed and expressed successfully.Publications1. Heinrich AC, Pelanda R, Klingmüller U. A mouse model for visualization and conditional mutations in the erythroid lineage[J]. Blood. 2004 Aug 1;104(3):659-66. [PMID:15090451]2.Akashi K, Traver D, Miyamoto T, Weissman IL. A clonogenic common myeloid progenitor that gives rise to all myeloid lineages[J]. Nature. 2000 Mar 9;404(6774):193-7.[PMID: 10724173]3. Zhang H, Wang S, Liu D, Gao C, Han Y, Guo X, Qu X, Li W, Zhang S, Geng J, Zhang L, Mendelson A, Yazdanbakhsh K, Chen L, An X. EpoR-tdTomato-Cre mice enable identification of EpoR expression in subsets of tissue macrophages and hematopoietic cells[J]. Blood. 2021 Nov 18;138(20):1986-1997. [PMID: 34098576]4.Parker MP, Peterson KR. Mouse Models of Erythropoiesis and Associated Diseases[J]. Methods Mol Biol. 2018;1698:37-65. PMID: 290760835. Peterson KR, Fedosyuk H, Zelenchuk L, Nakamoto B, Yannaki E, Stamatoyannopoulos G, Ciciotte S, Peters LL, Scott LM, Papayannopoulou T. Transgenic Cre expression mice for generation of erythroid-specific gene alterations[J]. Genesis. 2004 May;39(1):1-9. [PMID: 15124222]