C57BL/6-Nkp46tm1(icre)Bcgen/Bcgen • 110127
| Product name | B-Nkp46-iCre mice |
|---|---|
| Catalog number | 110127 |
| Strain name | C57BL/6-Nkp46tm1(icre)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 17086 |
| Official symbol | Ncr1(naturalcytotoxicity triggering receptor 1), NKp46; Ly94 |
| Chromosome | 7 |
| Application | This Ncr1iCre model is an efficient tool to study various gene functions when crossed with mice with different loxP site-flanked genes of interest, especially in studies of natural killer (NK) cells related signaling. |
ExpressionExpressed gene
iCre, improved crerecombinase, bacteriophage P1
Site of expression
The molecular isexpressed on resting and activated NK cells
Gene editing strategy
An IRES-iCre sequence cassette was placed between the coding sequence of exon 7 and 3'UTR of the Nkp46 gene using C57BL/6 embryonic stem cells. Neo cassette flanked by frt sites was inserted at least 300bp downstream of 3'UTR. This strain was maintained on a C57BL/6 genetic background.
Phenotype analysis
NKp46 þ ILC3 cells from Washflox/flox and Washflox/floxNcr1-Cre mice were in situ hybridized with probes against Ahr promoter, followed by staining with antibodies against WASH and Arid1a (upper panel). White arrow head indicates Ahr promoters colocalized with Arid1a. Percentages of cells with Arid1a colocalized Ahr promoter were calculated (lower panel). At least 200 NKp46 þ ILC3 cells were counted. Scale bar, 5 mm.(source: Xia P et al.,2017)
