SD-Oxttm1(icre)Bcgen/Bcgen • 210501
| Product name | B-Oxt-iCre rats |
|---|---|
| Catalog number | 210501 |
| Strain name | SD-Oxttm1(icre)Bcgen/Bcgen |
| Strain background | SD |
| NCBI gene ID | 18429 |
| Official symbol | Oxt (oxytocin), OT, Ox, Oxy |
| Chromosome | 2 |
| Application | This OxtiCre model is an efficient tool to study various gene functions when crossed with rats with different loxP site-flanked genes of interest, especially in studies of Oxytocin expressed in the PVN, SON and the olfactorycue. The development of social familiarity in rodents depends predominantly onolfactory cues and can critically influence reproductive success. |
ExpressionExpressed gene
iCre, improved crerecombinase, bacteriophage P1
Site of expression
supraoptic nucleus, paraventricularnuclei, the magnocellularsubnuclei of PVN
Gene editing strategy
A P2A-iCre sequence cassette was inserted upstream 3'UTR of the Oxt gene. This strain was maintained on a Sprague Dawley genetic background.
Phenotype analysis
(A) Schematic of AAV-Retro-DIO-YFP viral injection in OXT-Cre rats.(B) Immunostaining of YFP with OXT-NP on viral injected OXT-Cre rat brain slices. Co-localization of YFP with OXT-NP is indicated with white triangles.(C) Parvocellular neurons in the PVN were negative for YFP.(J) Schematic injection and chemogenetic inhibition of Magno-OXT neurons with AAV-Retro-DIO-hM4Di.(K) Representative images showing the viral infection of Magno-OXT neurons. Co-localization of mCherry with OXT-NP is indicated with white triangles.(source: Zhang B et al.,2021)
