Description
Phenotype Analysis
Experimental mice(B-Pdx-iCre mice (Mut;+), ROSA26-CAG-LSL-tdTomato(Mut;+) )were obtained by crossing B-Pdx-iCre mice with B-CAG-LSL-tdTomato mice.
1.1Analysis of Pancreas by FACS.
1.2Analysis of Small intestine by FACS.
1.3Analysis of kidney by FACS
1.4Analysis of lung by FACS
1.5Analysis of thymus by FACS
Pancreas and Small intestine were collected from wild-type C57BL/6 mice (+/+) and B-Pdx-iCre mice (Mut;+), ROSA26-CAG-LSL-tdTomato(Mut;+) mice, and analyzed by flow cytometry. TdTomato was only detectable in the B-Pdx-iCre mice (Mut;+), ROSA26-CAG-LSL-tdTomato(Mut;+) mice, and was not detectable in wild-type mice.WhileTdTomato was not detectable in kidney, lung and thymus,either wild-type C57BL/6 mice (+/+) or B-Pdx-iCre mice (Mut;+), ROSA26-CAG-LSL-tdTomato(Mut;+) mice.